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Literature summary for 3.2.1.193 extracted from

  • Zhong, F.L.; Ma, R.; Jiang, M.; Dong, W.W.; Jiang, J.; Wu, S.; Li, D.; Quan, L.H.
    Cloning and characterization of ginsenoside-hydrolyzing beta-glucosidase from Lactobacillus brevis that Ttransforms ginsenosides Rb1 and F2 into ginsenoside Rd and compound K (2016), J. Microbiol. Biotechnol., 26, 1661-1667 .
    View publication on PubMed
Search for more literature for 3.2.1.193

Application

Application Comment Organism
synthesis in pharmaceutical and commercial industries, this recombinant Bgy2 can be suitable for producting ginsenoside Rd and compound K Levilactobacillus brevis

Cloned(Commentary)

Cloned (Comment) Organism
gene bgy2, recombinant expression in Escherichia coli strain BL21(DE3) Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside F2 + H2O Levilactobacillus brevis
-
 compound K + D-glucose
-
 ?
ginsenoside F2 + H2O Levilactobacillus brevis KB290
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 compound K + D-glucose
-
 ?
ginsenoside Rb1 + H2O Levilactobacillus brevis
-
 ginsenoside Rd + D-glucose
-
 ?
ginsenoside Rb1 + H2O Levilactobacillus brevis KB290
-
 ginsenoside Rd + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis M5AXT3 isolated from a fermented food in Korea called kimchi
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Levilactobacillus brevis KB290 M5AXT3 isolated from a fermented food in Korea called kimchi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl-beta-D-glucopyranoside + H2O 93% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Levilactobacillus brevis 2-nitrophenol + D-glucopyranose
-
 ?
2-nitrophenyl-beta-D-glucopyranoside + H2O 93% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Levilactobacillus brevis KB290 2-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Levilactobacillus brevis 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Levilactobacillus brevis KB290 4-nitrophenol + D-glucopyranose
-
 ?
ginsenoside F2 + H2O
-
 Levilactobacillus brevis compound K + D-glucose
-
 ?
ginsenoside F2 + H2O
-
 Levilactobacillus brevis KB290 compound K + D-glucose
-
 ?
ginsenoside Rb1 + H2O
-
 Levilactobacillus brevis ginsenoside Rd + D-glucose
-
 ?
ginsenoside Rb1 + H2O
-
 Levilactobacillus brevis KB290 ginsenoside Rd + D-glucose
-
 ?
additional information beta-glucosidase Bgy2 cleaves the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. At optimal conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 are converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. No activity with 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside. Product identification by mass spectrometry Levilactobacillus brevis ?
-
 ?
additional information beta-glucosidase Bgy2 cleaves the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. At optimal conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 are converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. No activity with 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside. Product identification by mass spectrometry Levilactobacillus brevis KB290 ?
-
 ?

Synonyms

Synonyms Comment Organism
bgy2
-
 Levilactobacillus brevis
ginsenoside-hydrolyzing beta-glucosidase
-
 Levilactobacillus brevis
LVISKB_0241 locus name Levilactobacillus brevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant enzyme Levilactobacillus brevis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 about 95% of maximal activity at 20°C, about 30°C at 50°C, and 10% at 70°C Levilactobacillus brevis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 30 purified recombinnat enzyme, pH 7.0, completely stable at Levilactobacillus brevis
40
-
 purified recombinnat enzyme, pH 7.0, about 85% activity remaining Levilactobacillus brevis
50
-
 purified recombinnat enzyme, pH 7.0, complete inactivation Levilactobacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant enzyme Levilactobacillus brevis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8.5 activity range, profile overview, recombinant enzyme Levilactobacillus brevis

General Information

General Information Comment Organism
evolution the enzyme is a member of glycosyl hydrolase family 3, GH3 Levilactobacillus brevis